小鼠IL-21 ELISA试剂盒(Interleukin-21) (ab100707)

概述

  • 产品名称小鼠IL-21 ELISA试剂盒(Interleukin-21)
    参阅全部 IL21 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 6 pg/ml
  • 范围
    8.19 pg/ml - 2000 pg/ml
  • 复检

    89 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 98.65 87% - 109%
    Serum 93.57 83% - 104%
    Plasma 77.38 68% - 87%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-21 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-21 in serum, plasma and cell culture supernatants. (Mouse IL-21 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for mouse IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性A novel cytokine related to IL2 and IL15 was recently identified and designated IL21. IL21 has been found to be a powerful growth factor for naive B cells. The receptor for IL21 (IL21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL21 signaling. Both IL21R and the gc are necessary for the IL21 function. IL21 and its receptor activate JAKSTAT signaling pathway. IL21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL21 plays a role in the proliferation and maturation of NK, B and T cell populations.
  • 细胞定位Secreted
  • 别名
    • Cytokine CX2
    • DN 452A22.7
    • IL 21
    • Interleukin 21
    • Interleukin 21 isoform
    • Interleukin21
    • OTTMUSP00000007838
    • Za11
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-21 ELISA Kit (Interleukin-21) 图像

  • Representative standard curve using ab100707

  • Representative standard curve using ab100707

实验方案

Mouse IL-21 ELISA Kit (Interleukin-21) (ab100707)参考文献

ab100707 has not yet been referenced specifically in any publications.

Product Wall


We recommend a 2 fold dilution for plasma samples for use with all three of the mouse ELISA kits.

Thank you for your enquiry.

I can confirm that both the antibodies provided with ab100707 IL21 Mouse ELISA Kit are polyclonal.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"