小鼠IL-17F ELISA试剂盒(Interleukin-17F) (ab119538)

概述

  • 产品名称
    小鼠IL-17F ELISA试剂盒(Interleukin-17F)
    参阅全部 IL17F 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 4.2%
    批次间
    样品 n Mean SD CV%
    Overall 8 4.9%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    10 pg/ml
  • 范围
    62.5 pg/ml - 4000 pg/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-17F (Interleukin-17) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse IL-17F (Interleukin-17)concentrations in cell culture supernatant, serum and plasma (EDTA).


    IL-17F specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human IL-17F detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-17F captured on the plate.


    Get higher sensitivity in only 90 minutes with Mouse IL-17F ELISA Kit (ab204522) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab119538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119538.

实验方案

文献

ab119538 has not yet been referenced specifically in any publications.

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