小鼠IL-17F ELISA试剂盒(Interleukin-17F) (ab119538)

概述

  • 产品名称小鼠IL-17F ELISA试剂盒(Interleukin-17F)
    参阅全部 IL17F 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 4.2%
    批次间
    样品 n Mean SD CV%
    Overall 8 4.9%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    10 pg/ml
  • 范围
    62.5 pg/ml - 4000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-17F (Interleukin-17) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse IL-17F (Interleukin-17)concentrations in cell culture supernatant, serum and plasma (EDTA).


    IL-17F specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human IL-17F detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-17F captured on the plate.


    For higher sensitivity and reproducibility in only 90 minutes, we recommend Mouse IL-17F ELISA Kit (ab204522) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis.
  • 组织特异性Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.
  • 序列相似性Belongs to the IL-17 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CANDF6
    • Cytokine ML 1
    • Cytokine ML-1
    • IL 17F
    • IL 24
    • IL-17F
    • IL-24
    • Il17f
    • IL17F_HUMAN
    • Interleukin 17F
    • Interleukin 24
    • Interleukin-17F
    • Interleukin-24
    • ML 1
    • ML1
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab119538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-17F ELISA Kit (Interleukin-17F) 图像

  • Representative Standard Curve using ab119538.

实验方案

Mouse IL-17F ELISA Kit (Interleukin-17F) (ab119538)参考文献

ab119538 has not yet been referenced specifically in any publications.

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