小鼠IL-17B ELISA试剂盒(ab100703)
Key features and details
- Sensitivity: 12 pg/ml
- Range: 16.46 pg/ml - 12000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IL-17B ELISA试剂盒
参阅全部 IL-17B 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 12 pg/ml -
范围
16.46 pg/ml - 12000 pg/ml -
回收率
> 100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 149.4 139% - 161% Serum 118.9 104% - 128% Plasma 129.3 92% - 155% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s IL-17B Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-17B in serum (mouse IL-17B concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-17B coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17B present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-mouse IL-17B 2 vials IL-17B Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-17B Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. -
组织特异性
Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary. -
序列相似性
Belongs to the IL-17 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Cytokine like protein Zcyto7
- Cytokine Zcyto7
- IL 17B
see all -
数据库链接
- Entrez Gene: 56069 Mouse
- SwissProt: Q9QXT6 Mouse
- Unigene: 59313 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab100703 尚未被引用在任何文献中。