小鼠IL-17 ELISA试剂盒(Interleukin-17) (ab100702)

概述

  • 产品名称小鼠IL-17 ELISA试剂盒(Interleukin-17)
    参阅全部 IL17 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 6 pg/ml
  • 范围
    6.1 pg/ml - 600 pg/ml
  • 复检

    99 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 99.62 85% - 105%
    Serum 101.38 87% - 107%
    Plasma 97.47 84% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-17 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-17 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • 组织特异性Restricted to activated memory T-cells.
  • 序列相似性Belongs to the IL-17 family.
  • 翻译后修饰Found both in glycosylated and nonglycosylated forms.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CTLA 8
    • CTLA-8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17_HUMAN
    • Il17a
    • Interleukin 17A
    • Interleukin-17A
    • Interleukin17
    • Interleukin17A
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-17 ELISA Kit (Interleukin-17) 图像

  • Representative standard curve using ab100702

  • Representative standard curve using ab100702

实验方案

Mouse IL-17 ELISA Kit (Interleukin-17) (ab100702)参考文献

This product has been referenced in:
  • Dietert K  et al. mCLCA3 modulates IL-17 and CXCL-1 induction and leukocyte recruitment in murine Staphylococcus aureus pneumonia. PLoS One 9:e102606 (2014). Read more (PubMed: 25033194) »
  • Kotla S  et al. The transcription factor CREB enhances interleukin-17A production and inflammation in a mouse model of atherosclerosis. Sci Signal 6:ra83 (2013). Read more (PubMed: 24045154) »

See all 2 Publications for this product

Product Wall

When reconstituting your standard, we suggest that after it has been thoroughly defrosted, spin the vial down, reconstitute with assay diluent and then spin down again before use.
When making your standard dilutions, we recommend keeping everythin...

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Thank you for contacting us.
This ELISA kits is specific to IL17A. It does not recognize IL-17B, C, D or F.
Please let me know if you have any questions or comments.

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

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Thank you for your inquiry.

Unfortunately, for the antibodies in IL17 Mouse ELISA Kit ab100702 epitope mapping has not been performed, so we do not know the exact region the antibodies are binding to the mouse IL-17A protein. However, I can ...

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Following up my previous message, here are some recommendations for preparing cell and tissue lysates for use with this kit, though they are not specific for oviducts or lymph nodes: 1) Avoid using SDS or other strongly denaturing detergents. I...

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Thank you for contacting us. Can you please reply with a few details of the trouble you are having with the standard curve? For a positive control, I suggest isolating lymph nodes from a mouse, if possible. For the oviduct homogenate pr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"