小鼠IL-12 p70 ELISA试剂盒(Interleukin-12 p70) (ab119531)

概述

  • 产品名称小鼠IL-12 p70 ELISA试剂盒(Interleukin-12 p70)
    参阅全部 IL12 p70 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 7 8.3%
    批次间
    样品 n Mean SD CV%
    Overall 7 11%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    10 pg/ml
  • 范围
    15.6 pg/ml - 1000 pg/ml
  • 复检

    106 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 106 95% - 118%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    IL-20 p70 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-20 p70 in serum and cell culture supernatants.


    IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Biotin-conjugated anti-mouse IL-12 p70 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-12 p70 captured on the plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • 序列相似性Belongs to the IL-6 superfamily.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-12 p70 ELISA Kit (Interleukin-12 p70) 图像

  • Representative Standard Curve using ab119531.

实验方案

Mouse IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab119531)参考文献

ab119531 has not yet been referenced specifically in any publications.

Product Wall

GOOD

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Sample: Mouse Cell (Homogenised lung tissue)
Specification: Homogenised lung tissue
Type of Kit Used: ELISA
Positive control: Virally infected lung tissue.
Did you use the Abcam Kit protocol?: Yes
Additional data
Additional Notes: Overall, a good kit.
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提交于 Jan 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"