小鼠IL-10 ELISA试剂盒(Interleukin-10) (ab46103)

概述

  • 产品名称
    小鼠IL-10 ELISA试剂盒(Interleukin-10)
    参阅全部 IL10 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 8 556.8pg/ml 11.6 2%
    B 8 137.4pg/ml 6.8 4.9%
    批次间
    样品 n Mean SD CV%
    A 8 548.2pg/ml 12.1 2.2%
    B 8 133.6pg/ml 5.4 4%
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 检测时间
    3h 45m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-10 mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in mouse serum, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.

    This kit will recognize both endogenous and recombinant mouse IL-10.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab46103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46103

实验方案

文献

This product has been referenced in:
  • Li X  et al. Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation. EBioMedicine 8:72-82 (2016). Sandwich ELISA ; Mouse . Read more (PubMed: 27428420) »
  • Savvatis K  et al. Mesenchymal stromal cells but not cardiac fibroblasts exert beneficial systemic immunomodulatory effects in experimental myocarditis. PLoS One 7:e41047 (2012). Mouse . Read more (PubMed: 22815907) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for your enquiry. The antibodies used in the Murine IL10 ELISA kit are a goat polyclonal as capture antibody, and a rat monoclonal [B-C40] for detection. I hope this was the information you were looking for.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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