小鼠IL-10 ELISA试剂盒(Interleukin-10) (ab108870)

概述

  • 产品名称
    小鼠IL-10 ELISA试剂盒(Interleukin-10)
    参阅全部 IL10 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Tissue
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.03 ng/ml
  • 范围
    0.031 ng/ml - 2 ng/ml
  • 复检

    99.5 %

  • 检测时间
    5h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-10 (Interleukin-10) Mouse in vitro ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of IL-10 in plasma, serum, tissue extracts and cell culture supernatants.

    An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab108870

实验方案

文献

This product has been referenced in:
  • Rajan TS  et al. The secretome of periodontal ligament stem cells from MS patients protects against EAE. Sci Rep 6:38743 (2016). ELISA ; Mouse . Read more (PubMed: 27924938) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm that these three ELISA kits ab108870 ab100747 and ab100697 have been tested on mouse plasma and serum samples. (We would not recommend to test ...

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Merci de nous avoir contactés.

L'anticorps anti-IL10 du kit ab108870 est un polyclonal de lapin biotinylé dont l'immunogène est la séquence complète d'IL10 de souris.
La référence catalogue ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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