小鼠IL-10 ELISA试剂盒(Interleukin-10) (ab108870)

概述

  • 产品名称
    小鼠IL-10 ELISA试剂盒(Interleukin-10)
    参阅全部 IL10 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Tissue
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.03 ng/ml
  • 范围
    0.031 ng/ml - 2 ng/ml
  • 回收率

    99.5 %

  • 检测时间
    5h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-10 (Interleukin-10) Mouse in vitro ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of IL-10 in plasma, serum, tissue extracts and cell culture supernatants.

    An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab108870

实验方案

文献

This product has been referenced in:
  • Kang SJ  et al. Beneficial effects of dried pomegranate juice concentrated powder on ultraviolet B-induced skin photoaging in hairless mice. Exp Ther Med 14:1023-1036 (2017). Read more (PubMed: 28810554) »
  • Rajan TS  et al. The secretome of periodontal ligament stem cells from MS patients protects against EAE. Sci Rep 6:38743 (2016). ELISA ; Mouse . Read more (PubMed: 27924938) »

See all 2 Publications for this product

客户评价及客户问答

IL-10 ELISA kit

Average Below average 2/5 (Ease of Use)
Abreviews
Standard curve is ok. Worked well but it was overexposed in 7 minutes and it's written 20 minutes in the manual. Found some extra stuff in the kit which was not written in "material supplied" table.
Username

Miss. Sheelu Monga

Verified customer

提交于 Mar 27 2018

Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm that these three ELISA kits ab108870 ab100747 and ab100697 have been tested on mouse plasma and serum samples. (We would not recommend to test ...

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Merci de nous avoir contactés.

L'anticorps anti-IL10 du kit ab108870 est un polyclonal de lapin biotinylé dont l'immunogène est la séquence complète d'IL10 de souris.
La référence catalogue ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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