小鼠IL-10 ELISA试剂盒(Interleukin-10) (ab100697)

概述

  • 产品名称小鼠IL-10 ELISA试剂盒(Interleukin-10)
    参阅全部 IL10 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 45 pg/ml
  • 范围
    78.13 pg/ml - 5000 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 103.7 79% - 120%
    Serum 85.97 77% - 93%
    Plasma 92.2 71% - 134%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Mouse IL-10 (Interleukin-10) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 组织特异性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 序列相似性Belongs to the IL-10 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • TGIF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-10 ELISA Kit (Interleukin-10) 图像

  • Representative standard curve using ab100697

  • Representative standard curve using ab100697

实验方案

Mouse IL-10 ELISA Kit (Interleukin-10) (ab100697)参考文献

ab100697 has not yet been referenced specifically in any publications.

Product Wall

I have broken down the cross-reactivity information for each kit below:


· ab100549 IL10 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected ...

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Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm that these three ELISA kits ab108870 ab100747 and ab100697 have been tested on mouse plasma and serum samples. (We would not recommend to test ...

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Abreview for IL10 Mouse ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Mouse Cell (Homogenised lung tissue)
Specification: Homogenised lung tissue
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Virally infected lung tissue.
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Tissue samples were homogenised in T-PER (Thermo) and protein concentration measured at 5mg/ml with a BCA Assay. These could be diluted 1:5 - 1:100 in the Assay Diluent and incubated overnight at 4oC and the results were measurable with the kit.
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Abcam user community

Verified customer

提交于 Aug 17 2012

Thank you for your inquiry.

I can confirm that these kit are suitable for the use with serum samples that have been frozen.

I recommend to not to mix/compare samples thathave been frozen with samples that have not been frozen unless...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"