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|Cell culture extracts||102.34||90% - 111%|
|Tissue Extracts||98.26||89% - 109%|
Abcam’s IL-1 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 beta in cell lysates and tissue lysates.
This assay employs an antibody specific for mouse IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|组件||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|2X Cell Lysate Buffer||1 x 5ml|
|5X Assay Diluent||1 x 15ml|
|5X Sample Diluent Buffer||1 x 10ml|
|Biotinylated anti-mouse IL-1 beta||2 vials|
|IL-1 beta Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse IL-1 beta Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Dilution curves of mouse IL-1 beta (ab100705) and NIBSC standard (93/668). One ng of standard mouse IL-1 beta corresponds to 34.6 (+/-2.1) AU NIBSC 93/668. Background signal subtracted (duplicates; +/- SD).
IL-1 beta measured in mouse tissue lysates (0.02-0.3 mg x mL-1 protein tested, data expressed per
mg of extracted protein; duplicates +/- SD).
IL-1 beta measured in lysates from control cells, or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297), with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours (duplicates +/- SD).
Representative standard curve using ab100705
ab100705 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"