小鼠IL-1 beta ELISA试剂盒(Interleukin-1 beta) (ab100704)

概述

  • 产品名称小鼠IL-1 beta ELISA试剂盒(Interleukin-1 beta)
    参阅全部 IL1 beta 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    2.74 pg/ml - 2000 pg/ml
  • 复检

    99 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 98.64 89% - 110%
    Serum 99.36 89% - 108%
    Plasma 99.24 90% - 109%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-1 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 beta in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 序列相似性Belongs to the IL-1 family.
  • 结构域The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 细胞定位Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 别名
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-1 beta ELISA Kit (Interleukin-1 beta) 图像

  • Standard curve of msIL-1b in different diluents with background signal subtracted (duplicates; +/- SD).
  • Mouse IL-1b measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • IL-1b measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
  • IL-1b detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).
  • Representative standard curve using ab100704

  • Representative standard curve using ab100704

实验方案

Mouse IL-1 beta ELISA Kit (Interleukin-1 beta) (ab100704)参考文献

This product has been referenced in:
  • Hoppstädter J  et al. Glucocorticoid-induced leucine zipper: a critical factor in macrophage endotoxin tolerance. J Immunol 194:6057-67 (2015). Read more (PubMed: 25964494) »
  • Gaigé S  et al. Modification of energy balance induced by the food contaminant T-2 toxin: A multimodal gut-to-brain connection. Brain Behav Immun 37:54-72 (2014). Read more (PubMed: 24355099) »

See all 4 Publications for this product

Product Wall



It is possible to use the non-lysate kit ab100704 (IL-1 beta (Interleukin-1 beta) Mouse ELISA Kit) and use your own lysis buffers (non-denaturing, with a mild detergent like RIPA/NP40/Triton X). The cell lysate kit (ab100705) contains this bu...

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It is not clear if the kit is detecting IL-1 beta free or bound and we do not have any components or buffers that are specifically made to unbind IL-1 beta from the receptor.

Please see below available antibody specificity information:
C...

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I confirm this kit (ab100704) is compatible with heparin and EDTA.

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

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Thank you for your reply.


Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromi...

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Te pido disculpas por la espera.

He estado revisando los datos enviados con ayuda de mis compañeros del laboratorio. Los valores del estándar son débiles, pero no demasiado, alcanzando una densidad óptica de 0.875....

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Thank you for your patience.

I am still waiting on more information on ab108866.

But I am happy to provide the following information about ab100704 and ab100705:

The antibody pair used in the Mouse IL-1 beta Sandwich ELISA...

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Merci de nous avoir contactés.

Suite à notre conversation téléphonique, j’ai contacté le laboratoire. Ils m’ont répondu que diluent B fonctionne tout simplement mieux que le diluant A dans...

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Thank you very much for your call earlier this week and for your patience while I have been in touch with the lab.

The mouse IL1 beta ELISA kit detects the mature form of IL-1beta. It has not been tested for detection of the pro form, so whet...

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Merci de nous avoir contactés.

Le kit http://www.abcam.com/ab100704 est un kit d'ELISA sandwich, il contient donc 2 anticorps. L'anticorps de capture est un monoclonal de rat (numéro de clone non déterminé) et l'ant...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"