小鼠IgG2b, kappa单克隆抗体[7E10G10] -同型对照(ab170192)

概述

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituent: 99% HBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 克隆单克隆
  • 克隆编号7E10G10
  • 同种型IgG2b
  • 轻链类型kappa

    应用

    Our Abpromise guarantee covers the use of ab170192 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Flow Cyt Use a concentration of 1 µg/ml.
    ICC/IF Use a concentration of 1 µg/ml.

    Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control 图像

    • Immunofluorscent analysis of NIH 3T3, COS7, H9C2 and HeLa cells using ab170192 at 1µg/ml (top); no primary antibody negative control (middle), and ab14705 as a positive control (bottom). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

    • Flow cytometry experiments with 4% PFA fixed Hela (human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast)were performed with the IgG2b Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.

       

      The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

    • An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mLab170192 and detecting with Alexa Fluor® conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.

    Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)参考文献

    ab170192 has not yet been referenced specifically in any publications.

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