小鼠IgG2b, kappa单克隆抗体[7E10G10] -同型对照(ab170192)

概述

  • 产品名称
    小鼠IgG2b, kappa单克隆抗体[7E10G10] -同型对照
  • 特异性
    ab170192, mouse IgG2b Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
  • 经测试应用
    适用于: Flow Cyt, ICC/IFmore details
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.

    This product was previously marketed under the MitoSciences sub-brand.

    Alternative versions available:

    Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (ab221850)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium azide
    Constituent: 99% HBS
  • Concentration information loading...
  • 纯度
    Ammonium Sulphate Precipitation
  • 克隆
    单克隆
  • 克隆编号
    7E10G10
  • 同种型
    IgG2b
  • 轻链类型
    kappa

    应用

    Our Abpromise guarantee covers the use of ab170192 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Flow Cyt Use a concentration of 1 µg/ml.
    ICC/IF Use a concentration of 1 µg/ml.

    Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control 图像

    • Immunofluorscent analysis of NIH 3T3, COS7, H9C2 and HeLa cells using ab170192 at 1µg/ml (top); no primary antibody negative control (middle), and ab14705 as a positive control (bottom). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

    • Flow cytometry experiments with 4% PFA fixed Hela (human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast)were performed with the IgG2b Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.

       

      The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

    • An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mLab170192 and detecting with Alexa Fluor® conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.

    Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)参考文献

    ab170192 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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