小鼠IgG1 kappa [MOPC-21] (FITC) -同型对照(ab106163)

概述

  • 产品名称小鼠IgG1 kappa [MOPC-21] (FITC) -同型对照
    参阅全部 Mouse 亚类对照
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 共轭说明Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
  • 特异性ab106163 (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed Rat and Human tissues.
  • 经测试应用适用于: WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
  • 常规说明ab106163 is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.

性能

  • 别名
    • Mouse Isotype Control

应用

Our Abpromise guarantee covers the use of ab106163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

Mouse IgG1 kappa [MOPC-21] (FITC) - Isotype Control (ab106163)参考文献

This product has been referenced in:
  • Smed-Sörensen A  et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood 111:5037-46 (2008). Read more (PubMed: 18337560) »
  • Yates J  et al. The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol 19:785-99 (2007). Read more (PubMed: 17545278) »

See all 4 Publications for this product

Product Wall

Thank you for your reply.

The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please ...

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I have found one antibody which may be of use to you.

Please have a look at the datasheet of the Mouse antibody ab59498. This antibody has not been conjugated.

I hope this information has been of help. If you need any further inform...

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Thank you for your reply.

The MOUSE IgG1 antibody [MOPC-21] (FITC) (ab106163) is provided at a concentration of 1 mg/mL. This information can be found on the datasheet of ab106163 under the "Concentration" heading.

I hope ...

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Thank you for your reply.

I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-7...

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Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just ...

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Thank you very much for your interest in abcam antibodies. To our knowledge,these primary antibodies (ab27333, ab47095, ab30437 and ab11155)have not been tested in Western Blot and ICC/IF. They have been tested only in Flow cytometry as shown on the da...

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If the antibody datasheets only list "flow cytometry" in the Applications section, then that is the only application that has been tested. It is possible that the antibodies could also work for western blotting or ICC, but these applications ...

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A cells-only control with no antibody will show the contribution of autofluorescence of the cells themselves. Most people use this to set up the flow cytometer. An isotype control (cells incubated with the same isotype of antibody as the target-specifi...

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Thank you for contacting us. The current lot of the anti-CD162 FITC conjugate, ab88033, lot *****, is 0.01 mg/ml. The isotype control we were looking at, ab106163, is 1.0 mg/ml, so you will want to dilute that 100-fold to match the concentration o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"