小鼠IgG1,kappa单克隆抗体[15-6E10A7] -同型对照- BSA and Azide free (ab221848)
概述
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产品名称
小鼠IgG1,kappa单克隆抗体[15-6E10A7] -同型对照- BSA and Azide free
参阅全部 Coiled-coil domain-containing protein 113 亚类对照 -
特异性
This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. -
经测试应用
适用于: IHC-P, Flow Cyt, ELISA, ICC/IFmore details -
免疫原
Recombinant full length protein. ab221848 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.
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常规说明
This antibody clone is manufactured by Abcam.
ab221848 is a PBS only buffer formulated version of ab170190.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.5
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
克隆
单克隆 -
克隆编号
15-6E10A7 -
同种型
IgG1 -
轻链类型
kappa -
别名
- CCDC113
- Coiled-coil domain-containing protein 113 Gene names
- DKFZp434N1418
- HSPC065
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab221848于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration. PubMed: 26627833
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Flow Cyt |
Use a concentration of 1 µg/ml.
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-P
Use at an assay dependent concentration. PubMed: 26627833 |
Flow Cyt
Use a concentration of 1 µg/ml. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
图片
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This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# ab170190).
Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
实验方案
数据表及文件
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Datasheet download
文献 (4)
ab221848 被引用在 4 文献中.
- Cui Y et al. The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation. Sci Rep 6:23300 (2016). Flow Cyt . PubMed: 26996236
- Landa-Solís C et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank N/A:N/A (2015). PubMed: 26220398
- Ebisawa M et al. Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours. Vet J N/A:N/A (2015). IF . PubMed: 26364240
- Douglas DN et al. Oxidative stress attenuates lipid synthesis and increases mitochondrial fatty acid oxidation in hepatoma cells infected with hepatitis C virus. J Biol Chem N/A:N/A (2015). IHC-P . PubMed: 26627833