小鼠IGFBP5 ELISA试剂盒(ab208345)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 73 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IGFBP5 ELISA试剂盒
参阅全部 IGFBP5 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Mouse serum 5 3.6% 批次间 样品 n Mean SD CV% Mouse serum 3 2.4% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
73 pg/ml -
范围
125 pg/ml - 8000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 104 102% - 106% Serum 88 83% - 96% Hep Plasma 93 80% - 104% Cit plasma 98 92% - 102% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse
不与反应: Goat, Cow, Pig -
产品概述
Mouse IGFBP5 ELISA Kit (ab208345) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP5 protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP5 with 73 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Insulin-like growth factor-binding protein 5 (IGFBP5) is an IGF binding protein involved in prolonging its half-life, and has both inhibitory and stimulatory effects on IGFs. IGFBP5 can also alter the interaction of IGFs with their receptors.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse IGFBP5 Capture Antibody 1 x 600µl 10X Mouse IGFBP5 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR2 1 x 6ml Mouse IGFBP5 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
组织特异性
Osteosarcoma, and at lower levels in liver, kidney and brain. -
序列相似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
细胞定位
Secreted. - Information by UniProt
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别名
- IBP 5
- IBP-5
- IBP5
see all -
数据库链接
- Entrez Gene: 16011 Mouse
- SwissProt: Q07079 Mouse
- Unigene: 405761 Mouse
相关产品
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Alternative Versions
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ELISA kits
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Related Products
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of IGFBP5 were measured in duplicates, interpolated from the IGFBP5 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:600, plasma 1:800, and plasma (heparin) 1:800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP5 concentration was determined to be 2,058 ng/mL in serum, 3,670 ng/mL in plasma (citrate), and 3,865 ng/mL in plasma (heparin).
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The concentrations of IGFBP5 in rat serum were measured in duplicates, interpolated from the mouse IGFBP5 standard curves and corrected for sample dilution. Undiluted samples are rat serum 1:250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP5 concentration was determined to be 2691 ng/mL in rat serum.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab208345 被引用在 1 文献中.
- Huang X et al. Molecular characterization of a precision-cut rat liver slice model for the evaluation of antifibrotic compounds. Am J Physiol Gastrointest Liver Physiol 316:G15-G24 (2019). PubMed: 30406699