概述

  • 产品名称
    小鼠IGFBP5 ELISA试剂盒
    参阅全部 IGFBP5 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    4.12 pg/ml - 3000 pg/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 91.26 80% - 101%
    Serum 115.3 86% - 130%
    Plasma 95.11 83% - 102%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IGFBP5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP5 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse IGFBP5 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGFBP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get better reproducibility in only 90 minutes with Mouse IGFBP5 ELISA Kit (ab208345) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 功能
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 组织特异性
    Osteosarcoma, and at lower levels in liver, kidney and brain.
  • 序列相似性
    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • IBP 5
    • IBP-5
    • IBP5
    • IBP5_HUMAN
    • IGF binding protein 5
    • IGF BP5
    • IGF-binding protein 5
    • IGFBP 5
    • IGFBP-5
    • IGFBP5
    • Insulin like growth factor binding protein 5
    • Insulin-like growth factor-binding protein 5
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab100693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IGFBP5 ELISA Kit 图像

  • Representative Standard Curve using ab100693

实验方案

Mouse IGFBP5 ELISA Kit (ab100693)参考文献

This product has been referenced in:
  • Vijayan A  et al. IGFBP-5 enhances epithelial cell adhesion and protects epithelial cells from TGFß1-induced mesenchymal invasion. Int J Biochem Cell Biol 45:2774-85 (2013). Read more (PubMed: 24120850) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam.


Assay Diluent C is a very unique diluent so unfortunately we cannot release the formulation.


I apologize for any inconvenience this may cause. Please do not hesitate to contact us if you h...

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Thank you for contacting us. Yes, I can confirm that the Assay Diluent C is the same in both kits (ab100692, ab100693).   I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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