概述

  • 产品名称
    小鼠IGFBP2 ELISA试剂盒
    参阅全部 IGFBP2 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Serum 5 4.3%
    批次间
    样品 n Mean SD CV%
    Serum 3 4.9%
  • 样品类型
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    103.6 pg/ml
  • 范围
    179.68 pg/ml - 11500 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 97 92% - 102%
    Tissue Culture Media 95 94% - 96%
    Heparin Plasma 81 77% - 84%
    EDTA Plasma 104 96% - 111%
    Citrate Plasma 101 95% - 107%

  • 检测时间
    1h 30m
  • 实验步骤
    One step assay
  • 种属反应性
    与反应: Mouse, Rat
    不与反应: Rabbit, Guinea pig, Hamster, Cow, Dog, Pig
  • 产品概述

    Abcam’s IGFBP2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IGFBP2 protein in mouse serum, plasma, and cell culture supernatant.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 说明

    Insulin-like growth factor-binding protein 2 (IGFBP2) is an IGF binding protein involved in prolonging its half-life, and has both inhibitory and stimulatory effects on IGFs. IGFBP2 can also alter the interaction of IGFs with their receptors.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate (12 x 8 well strips)

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    10X Mouse IGFBP2 Capture Antibody 1 x 600µl
    10X Mouse IGFBP2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse IGFBP2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Substrate 1 x 12ml
  • 研究领域
  • 功能
    Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 序列相似性
    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • 结构域
    The C-terminus is required for IGF-binding and growth inhibition.
  • 翻译后修饰
    O-glycosylated.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • BP 2
    • BP2
    • IBP 2
    • IBP-2
    • IBP2
    • IBP2_HUMAN
    • IGF binding protein 2
    • IGF BP53
    • IGF-binding protein 2
    • IGFBP 2
    • IGFBP-2
    • IGFBP2
    • IGFBP53
    • Insulin like growth factor binding protein 2
    • Insulin like growth factor binding protein 2 36kDa
    • Insulin like growth factor-binding protein 2 precursor
    • Insulin-like growth factor-binding protein 2
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab207615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1.25%, plasma (citrate) 1%, plasma (heparin) 1% and plasma (EDTA) 1:160. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 492.8 ng/mL in serum, 325.6 ng/mL in plasma (citrate), 428.3 ng/mL in plasma (heparin) and 1247.2 ng/mL in plasma (EDTA).

  • The concentrations of IGFBP2 in rat serum were measured in duplicates, interpolated from the mouse IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are serum 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 357.6 ng/mL in rat serum.

实验方案

文献

ab207615 has not yet been referenced specifically in any publications.

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