小鼠IGFBP2 ELISA试剂盒(ab207615)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 103.6 pg/ml
- Range: 179.68 pg/ml - 11500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IGFBP2 ELISA试剂盒
参阅全部 IGFBP2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 5 4.3% 批次间 样品 n Mean SD CV% Serum 3 4.9% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
103.6 pg/ml -
范围
179.68 pg/ml - 11500 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 97 92% - 102% Tissue Culture Media 95 94% - 96% Hep Plasma 81 77% - 84% EDTA Plasma 104 96% - 111% Cit plasma 101 95% - 107% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse
不与反应: Cow, Pig -
产品概述
Mouse IGFBP2 ELISA Kit (ab207615) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP2 with 103.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Insulin-like growth factor-binding protein 2 (IGFBP2) is an IGF binding protein involved in prolonging its half-life, and has both inhibitory and stimulatory effects on IGFs. IGFBP2 can also alter the interaction of IGFs with their receptors.
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平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse IGFBP2 Capture Antibody 1 x 600µl 10X Mouse IGFBP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse IGFBP2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
序列相似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
结构域
The C-terminus is required for IGF-binding and growth inhibition. -
翻译后修饰
O-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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别名
- BP 2
- BP2
- IBP 2
see all -
数据库链接
- Entrez Gene: 16008 Mouse
- SwissProt: Q91VK7 Mouse
- SwissProt: P47877 Mouse
- Unigene: 141936 Mouse
相关产品
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Alternative Versions
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1.25%, plasma (citrate) 1%, plasma (heparin) 1% and plasma (EDTA) 1:160. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 492.8 ng/mL in serum, 325.6 ng/mL in plasma (citrate), 428.3 ng/mL in plasma (heparin) and 1247.2 ng/mL in plasma (EDTA).
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The concentrations of IGFBP2 in rat serum were measured in duplicates, interpolated from the mouse IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are serum 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 357.6 ng/mL in rat serum.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab207615 被引用在 5 文献中.
- Djeddi S et al. Multi-omics comparisons of different forms of centronuclear myopathies and the effects of several therapeutic strategies. Mol Ther 29:2514-2534 (2021). PubMed: 33940157
- Haywood NJ et al. IGFBP-1 in Cardiometabolic Pathophysiology-Insights From Loss-of-Function and Gain-of-Function Studies in Male Mice. J Endocr Soc 4:bvz006 (2020). PubMed: 32190801
- Greco CM et al. A non-pharmacological therapeutic approach in the gut triggers distal metabolic rewiring capable of ameliorating diet-induced dysfunctions encompassed by metabolic syndrome. Sci Rep 10:12915 (2020). PubMed: 32737396
- Han N et al. Downregulation of microRNA-873 attenuates insulin resistance and myocardial injury in rats with gestational diabetes mellitus by upregulating IGFBP2. Am J Physiol Endocrinol Metab 318:E723-E735 (2020). PubMed: 31910027
- Sheng X et al. Linear growth failure induced by systemic inflammation inhibiting IGF-1/IGFBP axis in rats with asymptomatic colitis. BMC Gastroenterol 19:96 (2019). PubMed: 31221091