概述

  • 产品名称
    小鼠IGFBP2 ELISA试剂盒
    参阅全部 IGFBP2 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 0.6 ng/ml
  • 范围
    0.823 ng/ml - 600 ng/ml
  • 回收率

    91 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 112.5 103% - 122%
    Serum 81.45 72% - 90%
    Plasma 80.22 73% - 92%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IGFBP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse IGFBP2 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Mouse IGFBP2 2 vials
    IGFBP2 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse IGFBP2 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 序列相似性
    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • 结构域
    The C-terminus is required for IGF-binding and growth inhibition.
  • 翻译后修饰
    O-glycosylated.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • BP 2
    • BP2
    • IBP 2
    • IBP-2
    • IBP2
    • IBP2_HUMAN
    • IGF binding protein 2
    • IGF BP53
    • IGF-binding protein 2
    • IGF-BP53
    • IGFBP-2
    • IGFBP2
    • IGFBP53
    • Insulin Like Growth Factor Binding Protein 2
    • Insulin like growth factor binding protein 2 36kDa
    • Insulin like growth factor-binding protein 2 precursor
    • Insulin-like growth factor-binding protein 2
    see all
  • 数据库链接

    应用

    Our Abpromise guarantee covers the use of ab100691 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent concentration.

    图片

    • Typical Standard Curve
      Typical Standard Curve

    实验方案

    文献

    This product has been referenced in:
    • Moya-Pérez A  et al. Hepatic molecular responses to Bifidobacterium pseudocatenulatum CECT 7765 in a mouse model of diet-induced obesity. Nutr Metab Cardiovasc Dis N/A:N/A (2013). Read more (PubMed: 23831006) »

    See 1 Publication for this product

    发表研究结果有使用 ab100691？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    3 reviews or Q&A

    10
    Votes: Highest First

    How should I dilute my normal mouse serum?

    Thank you for your inquiry.

    I heard back from the lab that the suggested dilution for normal serum/plasma is 2 - 10 fold. They do note that levels of the target protein may vary between different specimens. Optimal dilution factors for each s...

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    Customer needs another bottle of Diluent Bfor ab100691



    Sie können das Assay Diluent Bnun unter ab125050bestellen.

    Inquiry: Sehr geehrte Damen und Herren, bei der Durchführung des o.g. ELISA habe ich das 5xELISA Diluent B (Item E) versehentlich verschüttet. Da ich aber noch verbleibende Streifen habe, reicht das Diluent B nicht mehr für eine erneute ...

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    Leider verkaufen wir die einzelnen Kit Komponenten nicht separat, so dass ich Ihnen leider in Ihrem Fall nicht weiterhelfen kann.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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