概述

  • 产品名称
    小鼠IGF2 ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 1.5 ng/ml
  • 范围
    2.3 ng/ml - 150 ng/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IGF2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF2 in cell culture supernatants.

    This assay employs an antibody specific for mouse IGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Please note that ab100696 has only been validated with cell culture supernatant samples and cannot be used with plasma samples. It is also not validated for serum samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    1,000X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Mouse IGF2 (lyophilized) 2 vials
    IGF2 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse IGF2 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • 疾病相关
    Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS) [MIM:180860]. A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age.
  • 序列相似性
    Belongs to the insulin family.
  • 翻译后修饰
    O-glycosylated with core 1 or possibly core 8 glycans. Thr-96 is a minor glycosylation site compared to Thr-99.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • C11orf43
    • FLJ22066
    • FLJ44734
    • GRDF
    • IGF 2
    • IGF II
    • IGF-II
    • IGF2
    • IGF2_HUMAN
    • IGFII
    • INSIGF
    • Insulin like Growth Factor 2
    • Insulin like growth factor 2 (somatomedin A)
    • Insulin like growth factor II
    • Insulin like growth factor II precursor
    • Insulin like growth factor type 2
    • pp9974
    • Preptin
    • Putative insulin like growth factor II associated protein
    • Somatomedin A
    • Somatomedin-A
    • T3M 11 derived growth factor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Typical standard curve for ab100696

实验方案

文献

This product has been referenced in:
  • Ferrón SR  et al. Differential genomic imprinting regulates paracrine and autocrine roles of IGF2 in mouse adult neurogenesis. Nat Commun 6:8265 (2015). Read more (PubMed: 26369386) »
  • Moore SF  et al. Loss of the insulin receptor in murine megakaryocytes/platelets causes thrombocytosis and alterations in IGF signalling. Cardiovasc Res 107:9-19 (2015). Read more (PubMed: 25902782) »

See all 3 Publications for this product

客户评价及客户问答

The specificity information regarding the antibodies used in our Mouse IGFII ELISA kit can be seen below: Capture antibody: Detects mouse IGF­II in ELISAs and Western blots. In ELISAs, this antibody shows less than 6% cross­reactivity with reco...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册