概述

  • 产品名称小鼠IGF1 ELISA试剂盒
    参阅全部 IGF1 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Tissue Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 4 pg/ml
  • 范围
    2.74 pg/ml - 2000 pg/ml
  • 复检

    98 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 86.4 72% - 99%
    Serum 101.2 85% - 117%
    Plasma 106.6 85% - 114%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IGF1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IGF1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IGF1 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • 疾病相关Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • IBP1
    • IGF I
    • IGF IA
    • IGF IB
    • IGF-I
    • Igf1
    • IGF1_HUMAN
    • IGF1A
    • IGFI
    • IGFIA
    • Insulin like growth factor 1
    • Insulin like growth factor 1 (somatomedin C)
    • Insulin like growth factor IA
    • Insulin like growth factor IB
    • Insulin-like growth factor I
    • Mechano growth factor
    • MGF
    • OTTHUMP00000195080
    • OTTHUMP00000195081
    • OTTHUMP00000195082
    • OTTHUMP00000195083
    • OTTHUMP00000195084
    • Somatomedia C
    • Somatomedin C
    • Somatomedin-C
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IGF1 ELISA Kit 图像

  • Mouse IGF1 standard curve: mean of duplicates (+/- SD), performed in Diluent A

  • Mouse IGF1 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 500-4500 fold.

  • Representative Standard Curve using ab100695

  • Representative Standard Curve using ab100695

实验方案

Mouse IGF1 ELISA Kit (ab100695)参考文献

This product has been referenced in:
  • Hu L  et al. Low-frequency electrical stimulation attenuates muscle atrophy in CKD--a potential treatment strategy. J Am Soc Nephrol 26:626-35 (2015). Sandwich ELISA ; Mouse . Read more (PubMed: 25228359) »
  • Park JS & Höke A Treadmill exercise induced functional recovery after peripheral nerve repair is associated with increased levels of neurotrophic factors. PLoS One 9:e90245 (2014). Sandwich ELISA ; Mouse . Read more (PubMed: 24618564) »

See all 4 Publications for this product

Product Wall

Indeed our kits measures only free IGF-1, and the reason is because IGFBPs bind up IGF-1 and restrict it from binding the assay.  That is why some companies suggest a treatment step to limit/remove that binding and allow a total calculation of IGF-1 me...

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This kit is designed to measure free IGF1. As you stated, IGF-1 is usually bound to IGFBPs in the serum/plasma, so if you would like to measure total IGF1, it is advisable to release the analytes by performing an extraction procedure such as acid-ethan...

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We tested normal mouse serum/plasma levels ˜300 ng/ml (Serum/plasma-EDTA), ˜30ng/ml( plasma-Citrate and heparin).

IGF1 stability depends on how long they will be in travel. If it is a short period of time (1-2 hrs or so) ice would be sufficie...

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It would not be recommended to use Trizol for extraction as it contains Guanidinium thiocyanate which is a strong protein denaturant. Antibodies used in this kit recognize conformational epitopes of the target protein so it is important that the protei...

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The capture antibody used in this kit showed no cross-reactivity with human IGF-I and the detection antibody showed less than 0.5%. Thus, we do not believe the IGF1 Mouse ELISA kit will detect human IGF-I.

L’anticorps de capture de ce kit ne reconnait pas l’IGF-1 du rat. Pour l’anticorps de détection, la réactivé avec la protéine du rat n’a pas été testé donc malheureusement, ce kit...

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Jai contacté le laboratoire qui m’a confirmé que diluent A qui est utilisé pour des échantillons de sérum ou de plasma, est simplement un diluent et non pas un tampon d’extraction. Cette solution ne dissoc...

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Regarding the dilution, there is really no way to do a 1000-fold dilution and use < 30 ml of diluent testing 40 samples without doing a 2-step dilution. I’d give the below example of a 2-step dilution to conserve both sample and diluent:

...

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Thank you for your inquiry.


I can confirm that it is not necessary to determine the total protein in serum samples before using them with this kit. We are assuming the samples that are taken for one experiment all have roughly the same c...

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Abreview for IGF1 Mouse ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Rat Serum (Rat plasma)
Specification: Rat plasma
Amount of Sample: 2 ul used µg
Type of Kit Used: ELISA
Positive control: Different dilutions
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Although this is a Mouse IGF-1 kit, we used it to measure IGF-1 levels in rat plasma samples collected during harvest in EDTA tubes. We had success when samples were diluted 1:5000 prior to assay and the kit appears able to measure rat samples without a problem.

Small volume, serial dilutions of the samples were required to avoid running out of Assay Diluent A for these large dilutions (2ul in 98ul = 50x --> 2ul 50x in 198ul = 5000x)

We had some concern about the standard curve that was produced. We ran the kit on 3 separate occasions and each time the values for standards at low concentrations were quite variable and non-linear. Those of high concentration (>100pg/ml) were consistent and linear. The concentration of our samples (diluted 1:5000) fell in the high/linear portion of the curve.
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提交于 Oct 04 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"