小鼠IgE ELISA试剂盒(ab157718)
Key features and details
- Sensitivity: 1.83 ng/ml
- Range: 6.25 ng/ml - 400 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IgE ELISA试剂盒
参阅全部 IgE 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 10% -
样品类型
Serum, Plasma, Other biological fluids -
检测类型
Sandwich (quantitative) -
灵敏度
1.83 ng/ml -
范围
6.25 ng/ml - 400 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum > 85 % - % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s IgE Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgE in biological samples of mice.
In this assay the IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 100X HRP-conjugated anti-mouse IgE antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Mouse IgE Calibrator (Lyophilized) 1 vial Mouse IgE ELISA Microplate 1 unit Stop Solution 1 x 12ml -
研究领域
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相关性
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
细胞定位
Secreted -
别名
- Ig epsilon chain C region
- IgE
- IGHE
- immunoglobulin heavy constant epsilon
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数据库链接
- Entrez Gene: 380792 Mouse
- SwissProt: P06336 Mouse
图片
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab157718 被引用在 22 文献中.
- Zhang N et al. Bidirectional Transport of IgE by CD23 in the Inner Ear of Patients with Meniere's Disease. J Immunol 208:827-838 (2022). PubMed: 35046106
- Agrahari G et al. Inhibitory effects of superoxide dismutase 3 on IgE production in B cells. Biochem Biophys Rep 29:101226 (2022). PubMed: 35155837
- Song J & Wang J SIRT3 regulates bronchial epithelium apoptosis and aggravates airway inflammation in asthma. Mol Med Rep 25:N/A (2022). PubMed: 35234263
- Wei H et al. Activation of STAT6 by intranasal allergens correlated with the development of eosinophilic chronic rhinosinusitis in a mouse model. Int J Immunopathol Pharmacol 36:3946320221109529 (2022). PubMed: 35726645
- Ko HM et al. Rosa laevigata Attenuates Allergic Asthma Exacerbated by Water-Soluble PM by Downregulating the MAPK Pathway. Front Pharmacol 13:925502 (2022). PubMed: 35837279