概述

  • 产品名称小鼠I-TAC ELISA试剂盒(CXCL11)
    参阅全部 CXCL11 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 6 pg/ml
  • 范围
    5.487 pg/ml - 4000 pg/ml
  • 复检

    80 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 74.46 67% - 86%
    Serum 91.12 80% - 101%
    Plasma 75.6 68% - 88%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s I-TAC Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse I-TAC in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse I-TAC coated on a 96-well plate. Standards and samples are pipetted into the wells and I-TAC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse I-TAC antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-TAC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
  • 组织特异性High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • b R1
    • Beta R1
    • Beta-R1
    • betaR1
    • C X C motif chemokine 11
    • C-X-C motif chemokine 11
    • Chemokine (C-X-C motif) ligand 11
    • Chemokine C-X-C motif ligand 11
    • CXCL11
    • CXL11_HUMAN
    • H174
    • I TAC
    • I-TAC
    • Interferon gamma inducible protein 9
    • Interferon gamma-inducible protein 9
    • Interferon inducible T cell a chemoattractant I TAC
    • Interferon inducible T cell alpha chemoattractant
    • Interferon-inducible T-cell alpha chemoattractant
    • IP 9
    • IP-9
    • IP9
    • ITAC
    • MGC102770
    • SCYB11
    • SCYB9B
    • Small inducible cytokine B11
    • Small inducible cytokine subfamily B (Cys X Cys) member 11
    • Small inducible cytokine subfamily B (Cys X Cys) member 9B
    • Small inducible cytokine subfamily B, member 11
    • Small inducible cytokine subfamily B, member 9B
    • Small-inducible cytokine B11
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse I-TAC ELISA Kit (CXCL11) 图像

  • Representative standard curve using ab100716

  • Representative standard curve using ab100716

实验方案

Mouse I-TAC ELISA Kit (CXCL11) (ab100716)参考文献

This product has been referenced in:
  • Brenndörfer ED  et al. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy. J Immunol 192:1671-80 (2014). Sandwich ELISA ; Mouse . Read more (PubMed: 24442435) »

See 1 Publication for this product

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