小鼠HGF ELISA试剂盒(ab100686)

概述

  • 产品名称小鼠HGF ELISA试剂盒
    参阅全部 HGF 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.4 ng/ml
  • 范围
    0.614 ng/ml - 60 ng/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 111.8 98% - 123%
    Serum 111.4 98% - 129%
    Plasma 105.8 80% - 120%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s HGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse HGF coated on a 96- well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • 疾病相关Deafness, autosomal recessive, 39
  • 序列相似性Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • 别名
    • DFNB39
    • F TCF
    • Fibroblast derived tumor cytotoxic factor
    • Hepatocyte growth factor
    • Hepatocyte growth factor (hepapoietin A; scatter factor)
    • Hepatocyte growth factor beta chain
    • Hepatocyte growth factor precursor
    • Hepatopoietin A
    • Hepatopoietin-A
    • Hgf
    • HGF_HUMAN
    • HGFB
    • HPTA
    • Lung fibroblast derived mitogen
    • OTTHUMP00000161349
    • OTTHUMP00000206710
    • OTTHUMP00000206711
    • OTTHUMP00000206712
    • OTTHUMP00000206713
    • OTTHUMP00000206730
    • Scatter factor
    • SF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse HGF ELISA Kit 图像

  • Representative Standard Curve using ab100686

  • Representative Standard Curve using ab100686

实验方案

Mouse HGF ELISA Kit (ab100686)参考文献

ab100686 has not yet been referenced specifically in any publications.

Product Wall

The antibody pairs used in this kit recognize both the alpha and beta chains of mouse HGF

The antibody pair inab100686 recognizes an epitope located somewhere between aa 33-728 (which is whole sequence minus the signal peptide). Therefore the kit is expected to detect the pro (uncleaved) form, the heterodimeric form, the alpha subunit, and ...

Read More

Thank you for contacting us.

Both mouse and human HGF ELISA kits will detect total HGF.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your calls this week and for your patience.

I've heard back from my contact at the lab, and the standard is biologically active. Activity was measured as the ability to stimulate H-thymidine incorporation in 4MBr-5 monkey cells....

Read More

Thank you for contacting Abcam. Unfortunately, epitope mapping has not been performed for this kit so we do not know exactly where the antibodies are binding.  Therefore whether the kit will recognize HGF bound to the c-met receptor or inhibit c-met fr...

Read More

The best kit to use for tissue samples is ab100687 HGF Mouse ELISA Kit - 1 x 96 Well Plate. This is specifically meant for tissue samples. Both of the antibodies in the kit are polyclonal and considering that the homology between mouse and rat is hi...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"