小鼠HGF ELISA试剂盒(ab100686)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.614 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠HGF ELISA试剂盒
参阅全部 HGF 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.4 ng/ml -
范围
0.614 ng/ml - 60 ng/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 111.8 98% - 123% Serum 111.4 98% - 129% Plasma 105.8 80% - 120% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s HGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HGF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse HGF coated on a 96- well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse HGF 2 vials HGF Microplate (12 x 8 wells) 1 unit Recombinant Mouse HGF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. -
疾病相关
Deafness, autosomal recessive, 39 -
序列相似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
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别名
- DFNB39
- F TCF
- Fibroblast derived tumor cytotoxic factor
see all -
数据库链接
- Entrez Gene: 15234 Mouse
- SwissProt: Q08048 Mouse
- Unigene: 267078 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab100686 被引用在 2 文献中.
- Boldyreva MA et al. Transplantation of Adipose Stromal Cell Sheet Producing Hepatocyte Growth Factor Induces Pleiotropic Effect in Ischemic Skeletal Muscle. Int J Mol Sci 20:N/A (2019). PubMed: 31238604
- El Ouaamari A et al. Liver-derived systemic factors drive ß cell hyperplasia in insulin-resistant states. Cell Rep 3:401-10 (2013). PubMed: 23375376