小鼠heart组织裂解液- total protein (7 days) (ab7196)

概述

  • 产品名称小鼠heart组织裂解液- total protein (7 days)
    参阅全部 Heart 细胞裂解液
  • 描述
    小鼠heart总蛋白裂解液
  • 常规说明Source: Outbred NIH-Swiss Albino mice (7 days old)


    The mouse heart tissue was collected from seven (7) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用适用于: WBmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab7196 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent dilution. 10 µg to 20 µg per lane is recommended for mini gel.

    Mouse heart tissue lysate - total protein (7 days) (ab7196)参考文献

    ab7196 has not yet been referenced specifically in any publications.

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