小鼠heart normal组织裂解液- total protein (ab30291)

概述

  • 产品名称小鼠heart normal组织裂解液- total protein
    参阅全部 Heart 细胞裂解液
  • 描述
    小鼠heart总蛋白裂解液
  • 常规说明


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • 存储溶液Preservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • 裂解物说明Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab30291 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    Mouse heart normal tissue lysate - total protein (ab30291)参考文献

    ab30291 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. Unfortunately, I would not recommend using ab66470 as a positive control in Western blot: It is a synthetic peptide derived from within residues 600 - 700 of Human beta-cardiac myosin heavy chain. This peptide was used a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"