小鼠CXCL1 ELISA试剂盒(GRO alpha) (ab100717)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.614 pg/ml - 150 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠CXCL1 ELISA试剂盒(GRO alpha)
参阅全部 CXCL1/GRO alpha 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 1 pg/ml -
范围
0.614 pg/ml - 150 pg/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 123.5 115% - 139% Serum 89.3 78% - 97% Plasma 101.4 88% - 113% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s Mouse CXCL1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (GRO alpha) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL1 (GRO alpha) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CXCL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-mouse GRO alpha 2 vials GRO alpha Microplate (12 x 8 wells) 1 unit Recombinant mouse GRO alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
细胞定位
Secreted. - Information by UniProt
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别名
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all -
数据库链接
- Entrez Gene: 14825 Mouse
- SwissProt: P12850 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab100717 尚未被引用在任何文献中。