概述

  • 产品名称小鼠GM-CSF ELISA试剂盒
    参阅全部 GM-CSF 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 8 279.2pg/ml 14.7 5.2%
    B 8 62.5pg/ml 2.52 4%
    批次间
    样品 n Mean SD CV%
    A 7 271.9pg/ml 10.8 3.9%
    B 7 65.4pg/ml 4.3 6.5%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 9 pg/ml
  • 范围
    15.6 pg/ml - 500 pg/ml
  • 检测时间
    3h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s GM-CSF mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF in mouse serum, buffered solutions and cell culture medium.

    A monoclonal antibody specific for GM-CSF has been coated onto the wells of the microtiter strips provided. Samples, including standards of known GM-CSF concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for GM-CSF are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of GM-CSF present in the samples.

    This kit will recognize both endogenous and recombinant mouse GM-CSF.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • 序列相似性Belongs to the GM-CSF family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Colony stimulating factor
    • Colony Stimulating Factor 2
    • Colony stimulating factor 2 (granulocyte-macrophage)
    • Colony-stimulating factor
    • CSF
    • CSF 2
    • CSF2
    • CSF2_HUMAN
    • GM-CSF
    • GMCSF
    • Granulocyte Macrophage Colony Stimulating Factor
    • Granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • MGI1GM
    • Molgramostin
    • Pluripoietin-a
    • Sargramostim
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab46078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse GM-CSF ELISA Kit 图像

  • Representative Standard Curve using ab46078

实验方案

Mouse GM-CSF ELISA Kit (ab46078)参考文献

ab46078 has not yet been referenced specifically in any publications.

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