概述

  • 产品名称
    小鼠GM-CSF ELISA试剂盒
    参阅全部 GM-CSF 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 8 279.2pg/ml 14.7 5.2%
    B 8 62.5pg/ml 2.52 4%
    批次间
    样品 n Mean SD CV%
    A 7 271.9pg/ml 10.8 3.9%
    B 7 65.4pg/ml 4.3 6.5%
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 9 pg/ml
  • 范围
    15.6 pg/ml - 500 pg/ml
  • 检测时间
    3h 45m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s GM-CSF mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF in mouse serum, buffered solutions and cell culture medium.


    A monoclonal antibody specific for GM-CSF has been coated onto the wells of the microtiter strips provided. Samples, including standards of known GM-CSF concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for GM-CSF are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of GM-CSF present in the samples.


    This kit will recognize both endogenous and recombinant mouse GM-CSF.


    Get higher sensitivity in only 90 minutes with Mouse GM-CSF ELISA Kit (CSF2) (ab201276) from our SimpleStep ELISA® range.


     

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-GM-CSF Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    GM-CSF Microplate (12 x 8 well strips) 1 unit 2 units
    GM-CSF Standard (Lyophilized) Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 功能
    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • 序列相似性
    Belongs to the GM-CSF family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • Colony stimulating factor
    • Colony Stimulating Factor 2
    • Colony stimulating factor 2 (granulocyte-macrophage)
    • Colony-stimulating factor
    • CSF
    • CSF 2
    • CSF2
    • CSF2_HUMAN
    • GM-CSF
    • GMCSF
    • Granulocyte Macrophage Colony Stimulating Factor
    • Granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • MGI1GM
    • Molgramostin
    • Pluripoietin-a
    • Sargramostim
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab46078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46078

实验方案

文献

ab46078 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab46078.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册