小鼠Galectin 3 ELISA试剂盒(ab113343)

概述

  • 产品名称小鼠Galectin 3 ELISA试剂盒
    参阅全部 Galectin 3 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 6 pg/ml
  • 范围
    8.19 pg/ml - 2000 pg/ml
  • 复检

    > 100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 124.3 116% - 131%
    Serum 111.8 101% - 120%
    Plasma 99.08 90% - 107%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Galectin 3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Galectin 3 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse Galectin 3 coated on a 96- well plate. Standards and samples are pipetted into the wells and Galectin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Galectin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis By similarity. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Probably forms homo- or heterodimers. Interacts with DMBT1. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Contains 1 galectin domain.
  • 细胞定位Cytoplasmic, Nuclear and Secreted
  • 别名
    • 35 kDa lectin
    • Carbohydrate-binding protein 35
    • CBP 35
    • Gal-3
    • Galactose-specific lectin 3
    • Galactoside-binding protein
    • GALBP
    • Galectin-3
    • GALIG
    • IgE-binding protein
    • L-31
    • Laminin-binding protein
    • Lectin L-29
    • lectin, galactoside-binding, soluble, 3
    • LGALS2
    • Mac-2 antigen
    • MAC2
    • Macrophage galactose-specific lectin
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Galectin 3 ELISA Kit 图像

  • Representative Standard Curve using ab113343

  • Representative Standard Curve using ab113343

实验方案

Mouse Galectin 3 ELISA Kit (ab113343)参考文献

ab113343 has not yet been referenced specifically in any publications.

Product Wall

While this ELISA kit was neither designed nor validated with tissue lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. If you decide to test these kits with tissue lysate samples, we recom...

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Thank you for the clarification. This kit shows 10% reactivity with human. Please let me know if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"