小鼠Galectin 1 ELISA试剂盒(ab119595)

概述

  • 产品名称小鼠Galectin 1 ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    156 pg/ml - 10000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s mouse Galectin 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Galectin 1 in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Galectin 1 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Galectin 1 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Galectin 1 amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • 组织特异性Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • 序列相似性Contains 1 galectin domain.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • 14 kDa laminin-binding protein
    • 14 kDa lectin
    • Beta galactoside binding protein 14kDa
    • Beta-galactoside-binding lectin L-14-I
    • Gal-1
    • GAL1
    • Galaptin
    • Galectin-1
    • GBP
    • HBL
    • HLBP14
    • HPL
    • Lactose-binding lectin 1
    • Lectin galactoside-binding soluble 1
    • LEG1_HUMAN
    • LGALS1
    • Putative MAPK-activating protein PM12
    • S-Lac lectin 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Galectin 1 ELISA Kit 图像

  • Representative Standard Curve using ab119595

实验方案

Mouse Galectin 1 ELISA Kit (ab119595)参考文献

ab119595 has not yet been referenced specifically in any publications.

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