小鼠Fibronectin ELISA试剂盒(ab210967)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 251 pg/ml
- Range: 0.63 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
-
产品名称
小鼠Fibronectin ELISA试剂盒
参阅全部 Fibronectin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% serum 5 2.3% 批次间 样品 n Mean SD CV% serum 3 4.4% -
样品类型
Cell culture supernatant, Urine, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
251 pg/ml -
范围
0.63 ng/ml - 40 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 102 101% - 103% Urine 107 104% - 113% Serum 119 117% - 123% Hep Plasma 108 106% - 109% EDTA Plasma 107 103% - 112% Cit plasma 120 119% - 122% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse
不与反应: Cow -
产品概述
Mouse Fibronectin ELISA Kit (ab210967) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibronectin protein in cell culture supernatant, plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Fibronectin with 251 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
说明
Fibronectin is a large glycoprotein present in the extracellular matrix and circulating plasma. Fibronectin is important in many cell adhesion and migration related processes, including wound healing, embryogenesis, nerve regeneration and maintenance of cell shape.
-
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10 x 96 tests 1 x 384 tests 10X Mouse Fibronectin Capture Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Mouse Fibronectin Detector Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 4BR 1 x 6ml 10 x 6ml 1 x 6ml Mouse Fibronectin Lyophilized Recombinant Protein 2 vials 2 x 10 vials 2 vials Plate Seals 1 unit 1 x 10 units 1 unit Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml 1 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units 0 x 0 unit Stop Solution 1 x 12ml 1 x 120ml 2 x 12ml TMB Development Solution 1 x 12ml 1 x 120ml 2 x 12ml -
研究领域
-
功能
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
组织特异性
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
疾病相关
Glomerulopathy with fibronectin deposits 2 -
序列相似性
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
发展阶段
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
翻译后修饰
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
细胞定位
Secreted, extracellular space, extracellular matrix. - Information by UniProt
-
别名
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
see all -
数据库链接
- Entrez Gene: 14268 Mouse
- SwissProt: P11276 Mouse
相关产品
-
Alternative Versions
图片
-
Example of mouse Fibronectin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentration of native Fibronectin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:4,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
-
Background-subtracted data values (mean +/- SD) are graphed.
-
The concentrations of Fibronectin were measured in duplicates, interpolated from the Fibronectin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000, plasma (citrate) 1:4,000, plasma (EDTA) 1: 8,000, and plasma (heparin) 1: 10,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibronectin concentration was determined to be 100 µg/mL in serum, 172 µg/mL in plasma (citrate), 220 µg/mL in plasma (EDTA) and 211 µg/mL in plasma (heparin).
-
The concentrations of Fibronectin were measured in duplicates, interpolated from the Fibronectin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 5% and L929 supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibronectin concentration was determined to be 979 ng/mL in urine and 300 ng/mL in L929 supernatant.
-
L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Fibronectin was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the Fibronectin Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 300 ng/mL unstimulated, 2,209 ng/mL stimulated, and undetectable in media.
-
J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Fibronectin was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated J774A.1 and cell culture media. Measured values were interpolated from the Fibronectin Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 294 ng/mL unstimulated, 27.3 ng/mL stimulated, and undetectable in media.
-
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
To learn more about the advantages of recombinant antibodies see here.
-
To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab210967 被引用在 2 文献中.
- Soni H et al. Interleukin 1 beta-induced calcium signaling via TRPA1 channels promotes mitogen-activated protein kinase-dependent mesangial cell proliferation. FASEB J 35:e21729 (2021). PubMed: 34143493
- Yuan X et al. Construction of fibronectin conditional gene knock-out mice and the effect of fibronectin gene knockout on hematopoietic, biochemical and immune parameters in mice. PeerJ 8:e10224 (2020). PubMed: 33194415