概述

  • 产品名称小鼠Fibrinogen ELISA试剂盒
    参阅全部 Fibrinogen 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.7%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Plasma
  • 检测类型Competitive
  • 灵敏度
    = 0.63 µg/ml
  • 范围
    0.63 µg/ml - 40 µg/ml
  • 复检

    98 %

  • 检测时间
    3h 0m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Fibrinogen Mouse in vitro competitive ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of mouse fibrinogen in plasma.


    A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.


    For higher reproducibility in only 90 minutes, we recommend our SimpleStep ELISA® range made with recombinant monoclonal antibodies. Try Mouse Fibrinogen ELISA Kit (ab213478).

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • 组织特异性Plasma.
  • 疾病相关Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 序列相似性Contains 1 fibrinogen C-terminal domain.
  • 结构域A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • 翻译后修饰The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • FGA
    • Fib2
    • FIBA_HUMAN
    • Fibrinogen A alpha polypeptide
    • Fibrinogen alpha chain
    • Fibrinogen B alpha polypeptide
    • Fibrinogen beta chain
    • Fibrinogen G alpha polypeptide
    • Fibrinogen gamma chain
    • fibrinogen, B beta polypeptide
    • fibrinogen, G gamma polypeptide
    • fibrinogen, gamma polypeptide
    • Fibrinogen--alpha -polypeptide chain
    • Fibrinogen--beta -polypeptide chain
    • Fibrinogen--gamma-polypeptide chain
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab108844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

Mouse Fibrinogen ELISA Kit 图像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Fibrinogen measured in biological fluids showing quantity (ug) per mL of tested sample. Samples diluted 1-500 fold.

实验方案

Mouse Fibrinogen ELISA Kit (ab108844)参考文献

This product has been referenced in:
  • de la Rosa X  et al. Mannose-binding lectin promotes local microvascular thrombosis after transient brain ischemia in mice. Stroke 45:1453-9 (2014). Read more (PubMed: 24676774) »

See 1 Publication for this product

Product Wall

Yes, you will need to reconstitute the vial with 4 ml diluents and then dilute biotin solution 1:4 (4x) for use.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"