小鼠FGF23 ELISA试剂盒(ab213863)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠FGF23 ELISA试剂盒
参阅全部 FGF 23 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 35pg/ml 2.73 7.8% 2 16 216pg/ml 8.85 4.1% 3 16 449pg/ml 35.47 7.9% 批次间 样品 n Mean SD CV% 1 24 33pg/ml 2.77 8.4% 2 24 213pg/ml 12.78 6% 3 24 449pg/ml 35.47 7.9% -
样品类型
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
15.6 pg/ml - 1000 pg/ml -
检测时间
3h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
The Mouse FGF23 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213863) is designed for the quantitative detection of Mouse FGF23 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for FGF23 has been pre-coated onto 96-well plates. Standards (E.coli, Y25-V251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse FGF23 amount of sample captured in plate.
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说明
Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Adhesive Plate Seal 4 units 4 units Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Mouse FGF23 coated Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Mouse FGF23 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Mouse FGF23 standard 2 vials 2 vials Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究领域
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功能
Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. -
组织特异性
Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts). -
疾病相关
Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) [MIM:193100]. ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses.
Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. -
序列相似性
Belongs to the heparin-binding growth factors family. -
翻译后修饰
Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases.
O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. -
细胞定位
Secreted. Secretion is dependent on O-glycosylation. - Information by UniProt
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别名
- ADHR
- FGF-23
- Fgf23
see all -
数据库链接
- Entrez Gene: 64654 Mouse
- SwissProt: Q9EPC2 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab213863 被引用在 3 文献中.
- Willey JS et al. αKlotho decreases after reduced weight-bearing from both spaceflight and hindlimb unloading. NPJ Microgravity 8:18 (2022). PubMed: 35654945
- Clemens Z et al. The biphasic and age-dependent impact of klotho on hallmarks of aging and skeletal muscle function. Elife 10:N/A (2021). PubMed: 33876724
- Jia WH et al. Effects of fasting on the expression pattern of FGFs in different skeletal muscle fibre types and sexes in mice. Biol Sex Differ 11:9 (2020). PubMed: 32156311