概述

  • 产品名称小鼠FGF basic ELISA试剂盒
    参阅全部 FGF basic 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Tissue Lysate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 8 pg/ml
  • 范围
    18.8 pg/ml - 600 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 119.7 105% - 129%
    Serum 84.63 78% - 100%
    Plasma 83.26 76% - 99%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s FGF basic Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse FGF basic in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
  • 别名
    • Basic fibroblast growth factor
    • BFGF
    • FGF 2
    • FGF B
    • FGF2
    • FGF2 basic
    • FGFB
    • Fibroblast growth factor 2 (basic)
    • HBGF 2
    • HBGF2
    • HBGH 2
    • Heparin binding growth factor 2
    • Heparin binding growth factor 2 precursor
    • OTTMUSP00000046776
    • Prostatropin
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse FGF basic ELISA Kit 图像

  • Mouse FGF measured in biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 5-10 fold.

  • Mouse FGF standard curve: mean of duplicates (+/- SD), performed in Diluent A

  • Representative Standard Curve using ab100670.

  • Representative Standard Curve using ab100670.

实验方案

Mouse FGF basic ELISA Kit (ab100670)参考文献

This product has been referenced in:
  • Murillo MM  et al. RAS interaction with PI3K p110a is required for tumor-induced angiogenesis. J Clin Invest 124:3601-11 (2014). Read more (PubMed: 25003191) »
  • Paez-Gonzalez P  et al. Identification of distinct ChAT? neurons and activity-dependent control of postnatal SVZ neurogenesis. Nat Neurosci 17:934-42 (2014). Mouse . Read more (PubMed: 24880216) »

See all 2 Publications for this product

Product Wall

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I assume you are looking for a lysis buffer because you wish to test cell or tissue lysates samples with the kit? If so, please note that the ab100670 kit has not been validated with lysate samples, only serum, plasm...

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Although this kit was neither designed nor tested with cell or tissue lysate samples, this kit as well as our other sandwich ELISA kits are very accommodating to many different sample types so lysates samples shou...

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Thank you for contacting us. We do not currently sell any ELISA kits that are specifically designed for use on rat samples. After checking with the lab, our mouse and human ELISA kits for these targets will be unlikely to cross-react with rat, but have...

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Thank you very much for contacting us.

Unfortunately, epitope mapping of the two antibody pairs has not been performed, so we do not know the exact amino acid sequence recognised and if these regions are specific for human and mouse, respect...

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Thank you for your phone call today. I have heard back from my contact at the laboratory, and I was told that the ELISA plates come pre-blocked so a blocking step is not necessary.  I hope this information is helpful, but please let me know if y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"