概述

  • 产品名称
    小鼠Exodus 2 ELISA试剂盒
    参阅全部 Exodus 2 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.88 86% - 107%
    Serum 95.51 84% - 105%
    Plasma 94.29 83% - 102%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Exodus 2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Exodus 2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Exodus 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Exodus 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Exodus 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Exodus 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse Exodus 2 2 vials
    Exodus 2 Microplate (12 strips x 8 wells) 1 unit
    Recombinant Mouse Exodus 2 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
  • 别名
    • 6Ckine
    • Beta chemokine exodus 2
    • C-C motif chemokine 21
    • Chemokine (C-C motif) ligand 21
    • CKb9
    • ECL
    • Efficient Chemoattractant for Lymphocytes
    • Exodus2
    • MGC34555
    • OTTHUMP00000000526
    • OTTHUMP00000000527
    • OTTHUMP00000021303
    • SCYA 21
    • SCYA21
    • Secondary lymphoid tissue chemokine
    • SLC
    • Small inducible cytokine A21
    • Small inducible cytokine subfamily A (Cys-Cys), member 21
    • TCA 4
    • TCA4
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100667.

  • Representative Standard Curve using ab100667.

实验方案

文献

ab100667 has not yet been referenced specifically in any publications.

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