小鼠Epiregulin ELISA试剂盒(EREG) (ab100682)

概述

  • 产品名称小鼠Epiregulin ELISA试剂盒(EREG)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    83 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 102.6 94% - 110%
    Serum 74.19 68% - 89%
    Plasma 73.94 67% - 90%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 产品概述

    Abcam’s Epiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Epiregulin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse Epiregulin coated on a 96- well plate. Standards and samples are pipetted into the wells and Epiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Epiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Epiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Epiregulin is a member of the EGF-related growth factor family. It is a mitogen and has numerous roles including as an insulinotropic factor in pancreatic beta cell lines. It may also be involved in metastasis.
  • 别名
    • EPR
    • ER
    • EREG
    • Proepiregulin
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Epiregulin ELISA Kit (EREG) 图像

  • Representative Standard Curve using ab100682

  • Representative Standard Curve using ab100682

实验方案

Mouse Epiregulin ELISA Kit (EREG) (ab100682)参考文献

ab100682 has not yet been referenced specifically in any publications.

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The Epiregulin kit ab100682 is not suitable for Human samples since one of the antibody has shown no cross-reactivity with Human epiregulin, and unfortunately we do not have an ELISA kit for Human Epiregulin in our...

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The capture antibodyin this kit does cross-react with the human epiregulin, however the detection antibodyis not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"