概述

  • 产品名称小鼠Eotaxin ELISA试剂盒
    参阅全部 Eotaxin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    1.02 pg/ml - 100 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.25 83% - 104%
    Serum 94.95 83% - 104%
    Plasma 92.54 84% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Eotaxin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Eotaxin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Eotaxin coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Eotaxin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • C-C motif chemokine 11
    • CCL 11
    • CCL11
    • CCL11_HUMAN
    • Chemokine (C-C motif) ligand 11
    • Chemokine C C motif ligand 11
    • Chemokine CC Motif Ligand 11
    • Chemokine ligand 11
    • Eosinophil Chemotactic P
    • Eosinophil chemotactic protein
    • Eotaxin
    • Eotaxin 1
    • MGC22554
    • SCYA 11
    • SCYA11
    • Small inducible cytokine A11
    • Small inducible cytokine subfamily A (Cys Cys) member 11
    • Small inducible cytokine subfamily A member 11
    • Small-inducible cytokine A11
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Eotaxin ELISA Kit 图像

  • Representative standard curve using ab100680

  • Representative standard curve using ab100680

实验方案

Mouse Eotaxin ELISA Kit (ab100680)参考文献

This product has been referenced in:
  • Ren X  et al. FOXM1 promotes allergen-induced goblet cell metaplasia and pulmonary inflammation. Mol Cell Biol 33:371-86 (2013). Read more (PubMed: 23149934) »
  • Ci X  et al. Different Effects of Farrerol on an OVA-Induced Allergic Asthma and LPS-induced Acute Lung Injury. PLoS One 7:e34634 (2012). Read more (PubMed: 22563373) »

See all 2 Publications for this product

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