概述

  • 产品名称小鼠EGF ELISA试剂盒
    参阅全部 EGF 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    3.28 pg/ml - 800 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 112.4 83% - 107%
    Serum 88.33 75% - 101%
    Plasma 90.23 80% - 101%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s EGF (Epidermal growth factor) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse EGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse EGF coated on a 96- well plate. Standards and samples are pipetted into the wells and EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 组织特异性Expressed in kidney, salivary gland, cerebrum and prostate.
  • 疾病相关Hypomagnesemia 4
  • 序列相似性Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻译后修饰O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • Beta urogastrone
    • EGF
    • EGF_HUMAN
    • Epidermal growth factor
    • HOMG4
    • OTTHUMP00000219721
    • OTTHUMP00000219722
    • Pro epidermal growth factor
    • URG
    • Urogastrone
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse EGF ELISA Kit 图像

  • Representative Standard Curve using ab100679

  • Representative Standard Curve using ab100679

实验方案

Mouse EGF ELISA Kit (ab100679)参考文献

This product has been referenced in:
  • Good M  et al. Amniotic fluid inhibits Toll-like receptor 4 signaling in the fetal and neonatal intestinal epithelium. Proc Natl Acad Sci U S A 109:11330-5 (2012). Mouse . Read more (PubMed: 22733781) »

See 1 Publication for this product

Product Wall

Thanks for your phone call yesterday and for your questions about ab100679. This kit has not been tested with tissue lysates, but we do have some general guidelines for preparing tissue for our ELISA kits. The tissue should be lysed in a lysis ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"