小鼠E-Cadherin ELISA试剂盒(CDH1) (ab100678)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 4.12 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠E-Cadherin ELISA试剂盒(CDH1)
参阅全部 E Cadherin 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 6 pg/ml -
范围
4.12 pg/ml - 3000 pg/ml -
回收率
94 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 114.7 103% - 123% Serum 78.78 69% - 88% Plasma 88.93 76% - 95% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s E-Cadherin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse E-Cadherin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse E-Cadherin coated on a 96- well plate. Standards and samples are pipetted into the wells and E-Cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse E-Cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of E-Cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Mouse E-Cadherin ELISA Kit (ab197751) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse E-Cadherin 2 vials E Cadherin Microplate (12 x 8 wells) 1 unit Recombinant Mouse E-Cadherin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性
One of the epithelial cell adhesion molecules, E Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E Cadherin expression. Therefore, the expression of E Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm. -
别名
- Arc 1
- Cadherin 1
- cadherin 1 type 1 E-cadherin
see all -
数据库链接
- Entrez Gene: 12550 Mouse
- SwissProt: P09803 Mouse
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab100678 被引用在 1 文献中.
- Xiao Q et al. Functional role of matrix metalloproteinase-8 in stem/progenitor cell migration and their recruitment into atherosclerotic lesions. Circ Res 112:35-47 (2013). PubMed: 23071158