小鼠DKK1 ELISA试剂盒(Dickkopf-1) (ab113347)

概述

  • 产品名称小鼠DKK1 ELISA试剂盒(Dickkopf-1)
    参阅全部 DKK1 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 79.55 71% - 85%
    Serum 100.8 91% - 107%
    Plasma 91.38 83% - 100%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s DKK1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DKK1 in serum (mouse DKK1 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性DKK1 is an inhibitor of the Wnt signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
  • 别名
    • Dickkopf 1
    • Dickkopf homolog 1
    • Dickkopf related protein 1
    • DKK 1
    • hDkk 1
    • hDkk1
    • mDkk1
    • SK
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse DKK1 ELISA Kit (Dickkopf-1) 图像

  • Representative standard curve using ab113347

  • Representative standard curve using ab113347

实验方案

Mouse DKK1 ELISA Kit (Dickkopf-1) (ab113347)参考文献

ab113347 has not yet been referenced specifically in any publications.

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