概述

  • 产品名称小鼠CXCL5 ELISA试剂盒(GCP-2)
    参阅全部 CXCL5 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 35 pg/ml
  • 范围
    31.25 pg/ml - 2000 pg/ml
  • 复检

    91 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 83.94 74% - 94%
    Serum 98.05 86% - 106%
    Plasma 93.11 83% - 102%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s CXCL5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CXCL5 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for CXCL5 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应��适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
  • 别名
    • AMCF II
    • AMCFII
    • C X C motif chemokine 5
    • Chemokine (C X C motif) ligand 5
    • CXCL 5
    • ENA 78
    • ENA78
    • Epithelial derived neutrophil activating protein 78
    • GCP 2
    • LIX
    • Neutrophil activating peptide ENA 78
    • Neutrophil activating protein 78
    • SCYB5
    • Scyb6
    • Small inducible cytokine B5
    • Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78)
    • Small inducible cytokine subfamily B, member 15
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse CXCL5 ELISA Kit (GCP-2) 图像

  • Representative standard curve using ab100719

实验方案

Mouse CXCL5 ELISA Kit (GCP-2) (ab100719)参考文献

This product has been referenced in:
  • Lee CH  et al. Glutamine suppresses airway neutrophilia by blocking cytosolic phospholipase A(2) via an induction of MAPK phosphatase-1. J Immunol 189:5139-46 (2012). Mouse . Read more (PubMed: 23109722) »

See 1 Publication for this product

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