概述

  • 产品名称小鼠CTLA 4 ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    4.92 pg/ml - 1200 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 76.34 67% - 89%
    Serum 107.3 101% - 114%
    Plasma 104.2 100% - 110%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s CTLA4 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CTLA4 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse CTLA4 coated on a 96- well plate. Standards and samples are pipetted into the wells and CTLA4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CTLA4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CTLA4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab113346 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent concentration.

    Mouse CTLA 4 ELISA Kit 图像

    • Representative Standard Curve using ab113346

    • Representative Standard Curve using ab113346

    实验方案

    Mouse CTLA 4 ELISA Kit (ab113346)参考文献

    ab113346 has not yet been referenced specifically in any publications.

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