概述

  • 产品名称
    小鼠Complement C3 ELISA试剂盒
    参阅全部 C3 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 10%
  • 样品类型
    Serum, Plasma, Other biological fluids, Cell Lysate
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    1.658 ng/well
  • 范围
    3.13 ng/ml - 200 ng/ml
  • 复检

    > 85 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum > 85 % - %

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 产品概述

    Abcam's Complement C3 Mouse ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Complement C3 in mouse biological samples.

    In this assay the Complement C3 present in samples reacts with the anti- Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti- Complement C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 功能
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    Acylation stimulating protein (ASP): adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for GPR77. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of GPR77.
  • 组织特异性
    Plasma. The acylation stimulating protein (ASP) is expressed in adiopocytes and released into the plasma during both the fasting and postprandial periods.
  • 疾病相关
    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
    Note=Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
  • 序列相似性
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 翻译后修饰
    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • Acylation stimulating protein cleavage product
    • AHUS5
    • ARMD9
    • ASP
    • C3
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
    • C3a
    • C3a anaphylatoxin
    • C3adesArg
    • C3b
    • CO3_HUMAN
    • Complement C3
    • Complement C3c alpha'' chain fragment 2
    • Complement component 3
    • Complement component C3
    • Complement component C3a
    • Complement component C3b
    • CPAMD1
    • HSE-MSF
    • Prepro C3
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab157711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Complement C3 ELISA Kit 图像

  • Complement C3 measured in tissue extracts (total protein concentration: Ms liver - 17.46 mg/mL, Ms kidney - 15.6 mg/mL; prepared in RIPA buffer with protease inhibitor) and cell culture supernatants showing quantity (ng) per mL of tested sample. Tissue samples were diluted 100-10000 fold. Supernatants were diluted 1-10 fold.

  • Complement C3 measured in biological fluids of healthy species showing quantity (ng) per mL of tested sample. Samples were diluted 8000-128000 fold.

  • Representative standard curve using ab157711 Complement C3 Mouse ELISA kit

实验方案

Mouse Complement C3 ELISA Kit (ab157711)参考文献

This product has been referenced in:
  • Murphy S  et al. Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy. Int J Mol Med 39:1357-1370 (2017). ELISA . Read more (PubMed: 28440464) »
  • Murayama MA  et al. CTRP6 is an endogenous complement regulator that can effectively treat induced arthritis. Nat Commun 6:8483 (2015). Read more (PubMed: 26404464) »

See all 2 Publications for this product

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