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|Serum||> 85||% - %|
Abcam's Complement C3 Mouse ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Complement C3 in mouse biological samples.
In this assay the Complement C3 present in samples reacts with the anti- Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti- Complement C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|组件||1 x 96 tests|
|100X HRP-conjugated anti-mouse Complement C3 antibody||1 x 150µl|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|Chromogen Substrate Solution||1 x 12ml|
|Mouse Complement C3 Calibrator||1 vial|
|Mouse Complement C3 ELISA Microplate||1 unit|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab157711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Complement C3 measured in tissue extracts (total protein concentration: Ms liver - 17.46 mg/mL, Ms kidney - 15.6 mg/mL; prepared in RIPA buffer with protease inhibitor) and cell culture supernatants showing quantity (ng) per mL of tested sample. Tissue samples were diluted 100-10000 fold. Supernatants were diluted 1-10 fold.
Complement C3 measured in biological fluids of healthy species showing quantity (ng) per mL of tested sample. Samples were diluted 8000-128000 fold.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"