小鼠CD40L ELISA试剂盒(ab119517)
Key features and details
- Sensitivity: 0.14 ng/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠CD40L ELISA试剂盒
参阅全部 TRAP/CD40L 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 8 6.5% 批次间 样品 n Mean SD CV% Overall 8 11.1% -
样品类型
Cell culture supernatant, Serum -
检测类型
Sandwich (quantitative) -
灵敏度
0.14 ng/ml -
范围
0.31 ng/ml - 20 ng/ml -
回收率
94 %
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实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s CD40L Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse CD40L concentrations in cell culture supernatant and serum.
CD40L specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated CD40L detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD40L captured on the plate.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-mouse CD40L monoclonal antibody 1 x 70µl Microplate coated with monoclonal antibody to mouse CD40L (12 x 8 wells) 1 unit Mouse CD40L Standard lyophilized (40 ng /mL upon reconstitution) 2 vials Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究领域
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功能
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
组织特异性
Specifically expressed on activated CD4+ T-lymphocytes. -
疾病相关
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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别名
- CD 40L
- CD154
- CD40 antigen ligand
see all -
数据库链接
- Entrez Gene: 21947 Mouse
- SwissProt: P27548 Mouse
- Unigene: 4861 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab119517 被引用在 4 文献中.
- Fihurka O et al. The Memory Benefit to Aged APP/PS1 Mice from Long-Term Intranasal Treatment of Low-Dose THC. Int J Mol Sci 23:N/A (2022). PubMed: 35457070
- Lacy M et al. Cell-specific and divergent roles of the CD40L-CD40 axis in atherosclerotic vascular disease. Nat Commun 12:3754 (2021). PubMed: 34145241
- Pototskiy E et al. Downregulation of CD40L-CD40 attenuates seizure susceptibility and severity of seizures. Sci Rep 11:17262 (2021). PubMed: 34446808
- Thomas S et al. Development of a new fusion-enhanced oncolytic immunotherapy platform based on herpes simplex virus type 1. J Immunother Cancer 7:214 (2019). PubMed: 31399043