小鼠CD40 ELISA试剂盒(ab100674)

概述

  • 产品名称小鼠CD40 ELISA试剂盒
    参阅全部 CD40 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    2.06 pg/ml - 1500 pg/ml
  • 复检

    86 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 84.23 74% - 93%
    Serum 77.26 71% - 87%
    Plasma 76.68 69% - 87%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s CD40 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD40 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse CD40 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
  • 别名
    • AI326936
    • B cell associated molecule
    • B cell associated molecule CD40
    • B cell surface antigen CD40
    • Bp50
    • CD 40
    • CD40 antigen
    • CD40 antigen (TNF receptor superfamily member 5)
    • CD40 molecule
    • CD40 molecule TNF receptor superfamily member 5
    • CD40 protein
    • CD40 type II isoform
    • CD40L receptor
    • CDw40
    • GP39
    • HIGM1
    • IGM
    • MGC9013
    • Nerve growth factor receptor related B lymphocyte activation molecule
    • NFRSF5
    • OTTHUMP00000031699
    • OTTHUMP00000031700
    • p50
    • T BAM
    • TBAM
    • TNF receptor superfamily member 5
    • TNFRSF5
    • TRAP
    • Tumor necrosis factor receptor superfamily member 5
    • Tumor necrosis factor receptor superfamily member 5 precursor
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab100674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse CD40 ELISA Kit 图像

  • Representative Standard Curve using ab100674

  • Representative Standard Curve using ab100674

实验方案

Mouse CD40 ELISA Kit (ab100674)参考文献

ab100674 has not yet been referenced specifically in any publications.

Product Wall

Abreview for CD40 Mouse ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Mouse Serum (Also urine samples)
Specification: Also urine samples
Amount of Sample: 50 microliter µg
Type of Kit Used: ELISA
Positive control: Provided in the kit and positive serum samples
Did you use the Abcam Kit protocol?: Yes
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Abcam user community

Verified customer

提交于 Feb 12 2013

Thank you for your email.
This information is enough at the moment. I will contact you if I have any more question. Just to let you the Abreview will be processed next week because the person who is responsible for this is away at a conference.
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Expiry date: 09-01-2013
Submitting your protocol and data using our Abreview system will take about 10 minutes. To find out more abou...

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Thank you for your interest in Abcam's CD40 Mouse ELISA kit ab100674. Regarding a suggested dilution range for serum, we do not have any references currently available. We have not specified a range as this can vary significantly depending on the s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"