小鼠CD31 Matched抗体Pair试剂盒(ab212065)

概述

  • 产品名称小鼠CD31 Matched抗体Pair试剂盒
    参阅全部 CD31 试剂盒
  • 检测方法Colorimetric
  • 检测类型ELISA set
  • 范围
    31 pg/ml - 2000 pg/ml
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse CD31. 


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification. 


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab204527) which uses the same antibody pair. 


    This kit contains sufficient materials to perform ten 96-well ELISAs if using the assay procedure described in the booklet.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • 经测试应用适用于: ELISAmore details
  • 平台Reagents

性能

  • 功能Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 组织特异性Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 序列相似性Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻译后修饰Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 细胞定位Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 别名
    • Adhesion molecule
    • CD31
    • CD31 antigen
    • CD31 EndoCAM
    • EndoCAM
    • FLJ34100
    • FLJ58394
    • GPIIA
    • GPIIA'
    • PECA1
    • PECA1_HUMAN
    • PECAM
    • Pecam 1
    • PECAM 1 CD31 EndoCAM
    • PECAM-1
    • Pecam1
    • Platelet and endothelial cell adhesion molecule 1
    • Platelet endothelial cell adhesion molecule
    • Platelet/endothelial cell adhesion molecule 1
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab212065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Mouse CD31 Matched Antibody Pair Kit 图像

  • Standard calibration curve. Background subtracted values are graphed.

实验方案

Mouse CD31 Matched Antibody Pair Kit (ab212065)参考文献

ab212065 has not yet been referenced specifically in any publications.

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