小鼠CD30 ELISA试剂盒(ab113341)

概述

  • 产品名称小鼠CD30 ELISA试剂盒
    参阅全部 CD30 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    12.29 pg/ml - 3000 pg/ml
  • 复检

    76 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 77.29 69% - 87%
    Serum 75.99 68% - 84%
    Plasma 74.88 67% - 83%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s CD30 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD30 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse CD30 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD30 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD30 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD30 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab113341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse CD30 ELISA Kit 图像

  • Representative Standard Curve using ab113341

  • Representative Standard Curve using ab113341

实验方案

Mouse CD30 ELISA Kit (ab113341)参考文献

ab113341 has not yet been referenced specifically in any publications.

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Vielen Dank für Ihren Anruf heute Morgen. Ich muss Ihnen leider bestätigen, dass die Klonnummer der in diesem Kit verwendeten Antikörper dem Betriebsgeheimnis unterliegt. Ich darf Ihnen aber weitergeben, dass der "Capture"- Antikörper monoklonal ist un...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"