小鼠BMP2 ELISA试剂盒(ab119582)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠BMP2 ELISA试剂盒
参阅全部 BMP2 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Other biological fluids, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s mouse BMP2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse BMP2 in bone tissue, cell culture supernatants and serum.
A BMP2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BMP2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse BMP2 amount of sample captured in plate.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-mouse BMP2 antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-mouse BMP2 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant mouse BMP2 standard 2 x 10ng 2 x 10ng Plate Seal 4 units 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究领域
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功能
Induces cartilage and bone formation. -
组织特异性
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. -
序列相似性
Belongs to the TGF-beta family. -
细胞定位
Secreted. - Information by UniProt
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别名
- BDA2
- BMP-2
- BMP-2A
see all -
数据库链接
- Entrez Gene: 12156 Mouse
- SwissProt: P21274 Mouse
- Unigene: 103205 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab119582 被引用在 8 文献中.
- Wang M et al. A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection. Bioact Mater 6:1318-1329 (2021). PubMed: 33210025
- Zhang F et al. Inflammatory macrophages facilitate mechanical stress-induced osteogenesis. Aging (Albany NY) 12:3617-3625 (2020). PubMed: 32096768
- Wu R et al. Electrospun Fibers Immobilized with BMP-2 Mediated by Polydopamine Combined with Autogenous Tendon to Repair Developmental Dysplasia of the Hip in a Porcine Model. Int J Nanomedicine 15:6563-6577 (2020). PubMed: 32982218
- Zhang H et al. MicroRNA-410 participates in the pathological process of postmenopausal osteoporosis by downregulating bone morphogenetic protein-2. Exp Ther Med 18:3659-3666 (2019). PubMed: 31602244
- Wang C et al. Bone morphogenetic protein-2 exhibits therapeutic benefits for osteonecrosis of the femoral head through induction of cartilage and bone cells. Exp Ther Med 15:4298-4308 (2018). PubMed: 29849774
- Oda Y et al. Bone marrow stromal cells from low-turnover osteoporotic mouse model are less sensitive to the osteogenic effects of fluvastatin. PLoS One 13:e0202857 (2018). PubMed: 30142209
- Dube PR et al. Evidence for constitutive bone morphogenetic protein-2 secretion by M1 macrophages: Constitutive auto/paracrine osteogenic signaling by BMP-2 in M1 macrophages. Biochem Biophys Res Commun 491:154-158 (2017). PubMed: 28711495
- Mai Z et al. Single bout short duration fluid shear stress induces osteogenic differentiation of MC3T3-E1 cells via integrin ß1 and BMP2 signaling cross-talk. PLoS One 8:e61600 (2013). PubMed: 23593489