小鼠BCA1 ELISA试剂盒(BLC) (ab100671)

概述

  • 产品名称
    小鼠BCA1 ELISA试剂盒(BLC)
    参阅全部 BCA1 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    1.37 pg/ml - 1000 pg/ml
  • 复检

    99 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 104.56 90% - 110%
    Serum 96.89 83% - 103%
    Plasma 97.65 84% - 104%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s BCA1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse BCA1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse BCA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and BCA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse BCA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BCA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    BCA Microplate (12 strips x 8 wells) 1 unit
    Biotinylated anti-Mouse BCA1 2 vials
    Recombinant Mouse BCA Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles.
  • 别名
    • ANGIE
    • ANGIE2
    • B cell attracting chemokine 1
    • B cell chemoattractant
    • B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1)
    • B lymphocyte chemoattractant
    • BCA 1
    • BLC
    • BLR1L
    • C X C motif chemokine 13
    • Chemokine (C X C motif) ligand 13
    • Chemokine (C X C motif) ligand 13 (B cell chemoattractant)
    • Chemokine CXC motif ligand 13
    • CXC chemokine BLC
    • CXCL13
    • SCYB13
    • Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant)
    • Small inducible cytokine B13
    • Small inducible cytokine subfamily B, member 13
    see all

应用

Our Abpromise guarantee covers the use of ab100671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100671

  • Representative standard curve using ab100671

实验方案

文献

ab100671 has not yet been referenced specifically in any publications.

客户评价及客户问答



The capture antibody in this sandwich ELISA is a rat monoclonal.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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