小鼠 B7-1 ELISA试剂盒(CD80) (ab119578)

概述

  • 产品名称小鼠 B7-1 ELISA试剂盒(CD80)
    参阅全部 CD80 试剂盒
  • 检测方法Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Other biological fluids, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    62.5 pg/ml - 4000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s mouse CTLA4 (CD80) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse CTLA4 in cell culture supernatants and serum.

    A CTLA4 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for CTLA4 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse CTLA4 amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • 组织特异性Expressed on activated B-cells, macrophages and dendritic cells.
  • 序列相似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • Activation B7-1 antigen
    • B7
    • BB1
    • CD80
    • CD80_HUMAN
    • CTLA-4 counter-receptor B7.1
    • T-lymphocyte activation antigen CD80
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse B7-1 ELISA Kit (CD80) 图像

  • Representative Standard Curve Using ab119578.

实验方案

Mouse B7-1 ELISA Kit (CD80) (ab119578)参考文献

ab119578 has not yet been referenced specifically in any publications.

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